Windows Vista 32-bit version (RTM and SP1) works fine with the ancient ScanWizard Pro version 3.09 software downloadable from microtek.com, even though the microtek site does not state it is compatible with Windows Vista. There is a 64-bit Windows driver for Microtek scanners (not available from microtek.com) that is floating around the Internet (http://www.planetamd64.com) that works with Windows Vista 64-bit version (SP1) but has issues with VIA chipset motherboards.